Automated 4-sample protein immunoassays using 3D-printed microfluidics

Authors

Karteek Kadimisetty, University of Connecticut
Andrew P. Spak, University of Connecticut
Ketki S. Bhalerao, University of Connecticut
Mohamed Sharafeldin, University of Connecticut
Islam M. Mosa, University of Connecticut
Norman H. Lee, The George Washington University
James F. Rusling, University of Connecticut

Document Type

Journal Article

Publication Date

8-28-2018

Journal

Analytical Methods

Volume

10

Issue

32

DOI

10.1039/c8ay01271g

Abstract

© The Royal Society of Chemistry. Low cost, miniaturized assay platforms that work with small sample volumes, high sensitivity and rapid detection will have high value in future biomolecular diagnostics. Herein we report an automated, 3D printed electrochemiluminescent (ECL) immunoarray integrated with a nanostructured pyrolytic graphite sheet (PGS) microwell chip configured to detect 2 proteins simultaneously from complex liquid samples with high sensitivity and selectivity. Assays are done in 18 min at cost of

APA Citation

Kadimisetty, K., Spak, A., Bhalerao, K., Sharafeldin, M., Mosa, I., Lee, N., & Rusling, J. (2018). Automated 4-sample protein immunoassays using 3D-printed microfluidics. Analytical Methods, 10 (32). http://dx.doi.org/10.1039/c8ay01271g