Document Type
Journal Article
Publication Date
2017
Journal
Parasites and Vectors
Volume
10
Inclusive Pages
606
DOI
10.1186/s13071-017-2513-x
Abstract
Background
Human hookworm larvae arrest development until they enter an appropriate host. This makes it difficult to access the larvae for studying larval development or host-parasite interactions. While there are in vivo and in vitro animal models of human hookworm infection, there is currently no human, in vitro model. While animal models have provided much insight into hookworm biology, there are limitations to how closely this can replicate human infection. Therefore, we have developed a human, in vitro model of the initial phase of hookworm infection using intestinal epithelial cell culture.
Results
Co-culture of the human hookworm Ancylostoma ceylanicum with the mucus-secreting, human intestinal epithelial cell line HT-29-MTX resulted in activation of infective third-stage larvae, as measured by resumption of feeding. Larvae were maximally activated by direct contact with fully differentiated HT-29-MTX intestinal epithelial cells. HT-29-MTX cells treated with A. ceylanicum larvae showed differential gene expression of several immunity-related genes.
Conclusions
Co-culture with HT-29-MTX can be used to activate A. ceylanicum larvae. This provides an opportunity to study the interaction of activated larvae with the human intestinal epithelium.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
APA Citation
Feather, C., Hawdon, J. M., & March, J. (2017). Ancylostoma ceylanicum infective third-stage larvae are activated by co-culture with HT-29-MTX intestinal epithelial cells. Parasites and Vectors, 10 (). http://dx.doi.org/10.1186/s13071-017-2513-x
Peer Reviewed
1
Open Access
1
Included in
Medical Immunology Commons, Medical Microbiology Commons, Parasitic Diseases Commons, Parasitology Commons, Tropical Medicine Commons
Comments
Reproduced with permission of BioMed Central Ltd. Parasites and Vectors