Document Type
Journal Article
Publication Date
5-2016
Journal
Scientific Reports
Volume
6
DOI
10.1038/srep26016
Abstract
Over past several years, the cold plasma-stimulated medium (PSM) has shown its remarkable anti-cancer capacity in par with the direct cold plasma irradiation on cancer cells or tumor tissues. Independent of the cold plasma device, PSM has noticeable advantage of being a flexible platform in cancer treatment. Currently, the largest disadvantage of PSM is its degradation during the storage over a wide temperature range. So far, to stabilize PSM, it must be remained frozen at −80 °C. In this study, we first reveal that the degradation of PSM is mainly due to the reaction between the reactive species and specific amino acids; mainly cysteine and methionine in medium. Based on this finding, both H2O2 in PSM and the anti-cancer capacity of PSM can be significantly stabilized during the storage at 8 °C and −25 °C for at least 3 days by using phosphate-buffered saline (PBS) and cysteine/methionine-free Dulbecco’s Modified Eagle Medium (DMEM). In addition, we demonstrate that adding a tyrosine derivative, 3-Nitro-L-tyrosine, into DMEM can mitigate the degradation of PSM at 8 °C during 3 days of storage. This study provides a solid foundation for the future anti-cancer application of PSM.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
APA Citation
Yan, D., Nourmohammadi, N., Bian, K., Murad, F., Sherman, J. H., & Keidar, M. (2016). Stabilizing the cold plasma-stimulated medium by regulating medium’s composition. Scientific Reports, 6 (). http://dx.doi.org/10.1038/srep26016
Peer Reviewed
1
Open Access
1
Comments
Reproduced with permission of Nature Publishing Group. Scientific Reports.