Integrated relationship of nasopharyngeal airway host response and microbiome associates with bronchiolitis severity

Authors

Michimasa Fujiogi, Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. fujiogi-stm@umin.ac.jp.
Yoshihiko Raita, Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Marcos Pérez-Losada, Computational Biology Institute, Department of Biostatistics and Bioinformatics, The George Washington University, Washington, DC, USA.
Robert J. Freishtat, Center for Genetic Medicine Research, Children's National Hospital, Washington, DC, USA.
Juan C. Celedón, Division of Pulmonary Medicine, Department of Pediatrics, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA, USA.
Jonathan M. Mansbach, Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
Pedro A. Piedra, Departments of Molecular Virology and Microbiology and Pediatrics, Baylor College of Medicine, Houston, TX, USA.
Zhaozhong Zhu, Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Carlos A. Camargo, Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Kohei Hasegawa, Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Document Type

Journal Article

Publication Date

8-30-2022

Journal

Nature communications

Volume

13

Issue

1

DOI

10.1038/s41467-022-32323-y

Abstract

Bronchiolitis is a leading cause of infant hospitalizations but its immunopathology remains poorly understood. Here we present data from 244 infants hospitalized with bronchiolitis in a multicenter prospective study, assessing the host response (transcriptome), microbial composition, and microbial function (metatranscriptome) in the nasopharyngeal airway, and associate them with disease severity. We investigate individual associations with disease severity identify host response, microbial taxonomical, and microbial functional modules by network analyses. We also determine the integrated relationship of these modules with severity. Several modules are significantly associated with risks of positive pressure ventilation use, including the host-type I interferon, neutrophil/interleukin-1, T cell regulation, microbial-branched-chain amino acid metabolism, and nicotinamide adenine dinucleotide hydrogen modules. Taken together, we show complex interplays between host and microbiome, and their contribution to disease severity.

APA Citation

Fujiogi, Michimasa; Raita, Yoshihiko; Pérez-Losada, Marcos; Freishtat, Robert J.; Celedón, Juan C.; Mansbach, Jonathan M.; Piedra, Pedro A.; Zhu, Zhaozhong; Camargo, Carlos A.; and Hasegawa, Kohei, "Integrated relationship of nasopharyngeal airway host response and microbiome associates with bronchiolitis severity" (2022). GW Authored Works. Paper 1422.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/1422

Department

Pediatrics