A new tubulin-binding protein

Document Type

Journal Article

Publication Date

6-15-1982

Journal

Biochemical and Biophysical Research Communications

Volume

106

Issue

3

DOI

10.1016/0006-291X(82)91768-5

Abstract

A new brain protein is described which forms an insoluble complex with tubulin, with concomitant stoichiometric hydrolysis of GTP. The complex contains a maximum of one tubulin-binding protein (MW 52,500) per two tubulin dimers. The tubulin-binding protein (TBP) does not compete with colchicine, but in the presence of microtubule-associated proteins tubulin appeared less accessible to it. Proteins such as TBP might sequester tubulin and thereby function either to inhibit indiscriminate polymerization, or to promote ordered nucleation by maintaining high local concentrations. © 1982.

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