NF-κB Regulates Intestinal Epithelial Cell and Bile Salt-Induced Migration after Injury

Authors

Eric D. Strauch, University of Maryland School of Medicine
Barbara L. Bass, University of Maryland School of MedicineFollow
Jaladanki N. Rao, University of Maryland School of Medicine
Jennifer A. Vann, University of Maryland School of Medicine
Jian Ying Wang, University of Maryland School of Medicine

Document Type

Journal Article

Publication Date

4-1-2003

Journal

Annals of Surgery

Volume

237

Issue

4

DOI

10.1097/00000658-200304000-00010

Abstract

Objective: To determine if NF-κB regulates intestinal epithelial cell migration and if it has a role during bile salt-induced migration. Summary Background Data: Mucosal restitution is an important repair modality in the gastrointestinal tract. The authors have shown that taurodeoxycholate (TDCA) increases intestinal epithelial cell migration. NF-κB regulates activation of a number of genes involved in inflammatory responses. Methods: Studies were conducted in IEC-6 cells. IκB protein expression was determined by Western blot analysis. Sequence-specific NF-κB binding activity was measured by EMSA shift assays and nuclear localization by immunohistochemistry. Cell migration was examined by using an in vitro model that mimics the early cell division-independent stages of epithelial restitution. Results: The process of cell migration over the wounded area was associated with a significant increase in NF-κB binding activity in IEC-6 cells. Immunohistochemistry revealed translocation of NF-κB into the nucleus. Western blot analysis showed that injury decreased IκB protein expression, inhibition of the binding activity by treatment with a specific NF-κB inhibitor, MG-132, inhibited cell migration during restitution. Further, exposure to TDCA at the physiologic concentration that induces intestinal epithelial cell migration increased NF-κB binding activity, induced NF-κB translocation into the nucleus, and decreased IκB protein expression. MG-132 also inhibits bile salt-induced cell migration. Conclusions: NF-κB regulates intestinal epithelial cell migration. Bile salts at physiologic concentrations increase cell migration by activation of NF-κB. These data show that oile salts may have a role in the maintenance of intestinal mucosal integrity.

APA Citation

Strauch, E., Bass, B., Rao, J., Vann, J., & Wang, J. (2003). NF-κB Regulates Intestinal Epithelial Cell and Bile Salt-Induced Migration after Injury. Annals of Surgery, 237 (4). http://dx.doi.org/10.1097/00000658-200304000-00010