Evaluation of Single Nucleotide Polymorphisms (SNPs) in the p53 binding protein 1 (TP53BP1) gene in breast cancer patients treated with breast-conserving surgery and whole-breast irradiation (BCS + RT)

Authors

Bruce G. Haffty, Rutgers Robert Wood Johnson Medical School at New Brunswick
Sharad Goyal, Rutgers Robert Wood Johnson Medical School at New Brunswick
Diptee Kulkarni, Rutgers Robert Wood Johnson Medical School at New Brunswick
Camille Green, Rutgers Robert Wood Johnson Medical School at New Brunswick
Alexi Vazquez, Rutgers Robert Wood Johnson Medical School at New Brunswick
Devora Schiff, Rutgers Robert Wood Johnson Medical School at New Brunswick
Meena S. Moran, Yale University
Qifeng Yang, Qilu Hospital of Shandong University
Shridar Ganesan, Rutgers Robert Wood Johnson Medical School at New Brunswick
Kim M. Hirsfield, Rutgers Robert Wood Johnson Medical School at New Brunswick

Document Type

Journal Article

Publication Date

6-1-2011

Journal

International Journal of Radiation Oncology Biology Physics

Volume

80

Issue

2

DOI

10.1016/j.ijrobp.2010.02.005

Keywords

Breast cancer; Breast-conserving surgery; Local recurrence, 53BP1; Single nucleotide polymorphism

Abstract

Purpose: TP53BP1 is a key component of radiation-induced deoxyribonucleic acid damage repair. The purpose of this study was to evaluate the significance of a known common single nucleotide polymorphism in this gene (rs560191) in patients treated with breast-conserving surgery and whole-breast irradiation (BCS + RT). Methods and Materials: The population consisted of 176 premenopausal women treated with BCS + RT (median follow-up, 12 years). Genomic deoxyribonucleic acid was processed by use of TaqMan assays. Each allele for rs560191 was either C or G, so each patient was therefore classified as CC, CG, or GG. Patients were grouped as GG if they were homozygous for the variant G allele or CC-CG if they carried at least one copy of the common C allele (CC or CG). Results: Of the 176 women, 124 (71%) were CC-CG and 52 (29%) were GG. The mean age was 44 years for GG vs. 38 years for CC-CG (p < 0.001). GG was more common in African-American women than white women (69% vs. 13%, p < 0.001) and more commonly estrogen receptor negative (70% vs. 49%, p = 0.02). There were no significant correlations of rs560191 with other critical variables. Despite the fact that GG patients were older, the 10-year rate of local relapses was higher (22% for GG vs. 12% for CC-CG, p = 0.04). Conclusions: This novel avenue of investigation of polymorphisms in radiation repair/response genes in patients treated with BCS + RT suggests a correlation to local relapse. Additional evaluation is needed to assess the biological and functional significance of these single nucleotide polymorphisms, and larger confirmatory validation studies will be required to determine the clinical implications. © 2011 Elsevier Inc.

APA Citation

Haffty, B., Goyal, S., Kulkarni, D., Green, C., Vazquez, A., Schiff, D., Moran, M., Yang, Q., Ganesan, S., & Hirsfield, K. (2011). Evaluation of Single Nucleotide Polymorphisms (SNPs) in the p53 binding protein 1 (TP53BP1) gene in breast cancer patients treated with breast-conserving surgery and whole-breast irradiation (BCS + RT). International Journal of Radiation Oncology Biology Physics, 80 (2). http://dx.doi.org/10.1016/j.ijrobp.2010.02.005