Document Type
Journal Article
Publication Date
7-29-2017
Journal
EBioMedicine
DOI
10.1016/j.ebiom.2017.07.019
Abstract
Latently human immunodeficiency virus (HIV)-infected cells are transcriptionally quiescent and invisible to clearance by the immune system. To demonstrate that the latency reversing agent vorinostat (VOR) induces a window of vulnerability in the latent HIV reservoir, defined as the triggering of viral antigen production sufficient in quantity and duration to allow for recognition and clearance of persisting infection, we developed a latency clearance assay (LCA). The LCA is a quantitative viral outgrowth assay (QVOA) that includes the addition of immune effectors capable of clearing cells expressing viral antigen. Here we show a reduction in the recovery of replication-competent virus from VOR exposed resting CD4 T cells following addition of immune effectors for a discrete period. Take home message: VOR exposure leads to sufficient production of viral protein on the cell surface, creating a window of vulnerability within this latent reservoir in antiretroviral therapy (ART)-suppressed HIV-infected individuals that allows the clearance of latently infected cells by an array of effector mechanisms.
APA Citation
Sung, J., Sholtis, K., Kirchherr, J., Kuruc, J., Gay, C., Nordstrom, J., Bollard, C., Archin, N., & Margolis, D. (2017). Vorinostat Renders the Replication-Competent Latent Reservoir of Human Immunodeficiency Virus (HIV) Vulnerable to Clearance by CD8 T Cells.. EBioMedicine, (). http://dx.doi.org/10.1016/j.ebiom.2017.07.019
Peer Reviewed
1
Open Access
1
Comments
Reproduced with permission of Elsevier B.V. eBioMedicine