Document Type
Journal Article
Publication Date
2017
Journal
Molecular Therapy - Methods and Clinical Development
Volume
5
Inclusive Pages
13-21
DOI
10.1016/j.omtm.2017.02.001
Abstract
Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Adoptive transfer of ex vivo-expanded virus-specific T cells from CB is both feasible and safe. However, the manufacturing process of these cells is complicated, lengthy, and labor-intensive. We have now developed a simplified method to manufacture a single culture of polyclonal multivirus-specific cytotoxic T cells in less than 30 days. It eliminates the need for a live virus or transduction with a viral vector, thus making this approach widely available and GMP-applicable to target multiple viruses. The use of overlapping PepMixes as a source of antigen stimulation enable expansion of the repertoire of the T cell product to any virus of interest and make it available as a third party “off the shelf” treatment for viral infections following transplantation.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
APA Citation
Dave, H., Luo, M., Blaney, J., Patel, S., Barese, C., Cruz, C. R., Shpall, E. J., Bollard, C. M., & Hanley, P. J. (2017). Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood. Molecular Therapy - Methods and Clinical Development, 5 (). http://dx.doi.org/10.1016/j.omtm.2017.02.001
Peer Reviewed
1
Open Access
1
Comments
Reproduced with permission of Elsevier B.V. Molecular Therapy - Methods and Clinical Development