The association of CD81 polymorphisms with alloimmunization in sickle cell disease

Authors

Zohreh Tatari-Calderone, George Washington University
Ryad Tamouza
Gama P. LeBouder
Ramita Dewan
Naomi L. Luban, George Washington UniversityFollow
Jacqueline Lasserre
Jacqueline Maury
Francois Lionnet
Rajagopal Krishnamoorthy
Robert Girot
Stanislav Vukmanovic, George Washington University

Document Type

Journal Article

Publication Date

2013

Journal

Clinical and Developmental Immunology

Inclusive Pages

Article ID 937846

Keywords

Anemia, Sickle Cell--genetics; Antigens, CD81--genetics; Isoantibodies--biosynthesis; Polymorphism, Single Nucleotide; Receptors, Nicotinic--genetic; rho GTP-Binding Proteins--genetics

Abstract

The goal of the present work was to identify the candidate genetic markers predictive of alloimmunization in sickle cell disease (SCD). Red blood cell (RBC) transfusion is indicated for acute treatment, prevention, and abrogation of some complications of SCD. A well-known consequence of multiple RBC transfusions is alloimmunization. Given that a subset of SCD patients develop multiple RBC allo-/autoantibodies, while others do not in a similar multiple transfusional setting, we investigated a possible genetic basis for alloimmunization. Biomarker(s) which predicts (predict) susceptibility to alloimmunization could identify patients at risk before the onset of a transfusion program and thus may have important implications for clinical management. In addition, such markers could shed light on the mechanism(s) underlying alloimmunization. We genotyped 27 single nucleotide polymorphisms (SNPs) in the CD81, CHRNA10, and ARHG genes in two groups of SCD patients. One group (35) of patients developed alloantibodies, and another (40) had no alloantibodies despite having received multiple transfusions. Two SNPs in the CD81 gene, that encodes molecule involved in the signal modulation of B lymphocytes, show a strong association with alloimmunization. If confirmed in prospective studies with larger cohorts, the two SNPs identified in this retrospective study could serve as predictive biomarkers for alloimmunization.

Comments

Reproduced with permission of Hindawi Publishing Corp.

Creative Commons License

This work is licensed under a Creative Commons Attribution 3.0 License.

APA Citation

Tatari-Calderone, Z., Tamouza, R., LeBouder, G.P., Dewan, R., Luban, N.L.C., Lasserre, J., Maury, J., Lionnet, F., Krishnamoorthy, R., Girot, R. & Vukmanovic, S. (2013). The association of CD81 polymorphisms with alloimmunization in sickle cell disease. Clinical and Developmental Immunology, article ID 937846.

Peer Reviewed

1

Open Access

1