"Downregulation of Estrogen Receptor Beta1 Expression in Sessile Serrat" by Mamoun Younes, Ayush Arora et al.
 

Downregulation of Estrogen Receptor Beta1 Expression in Sessile Serrated Adenomas

Document Type

Journal Article

Publication Date

11-1-2019

Journal

Annals of clinical and laboratory science

Volume

49

Issue

6

Abstract

© 2019 by the Association of Clinical Scientists, Inc. OBJECTIVE: CpG island methylator phenotype (CIMP)-positive colorectal cancers (CRC) and CRC with microsatellite instability (MSI) were reported to have a decreased expression of estrogen receptor, beta1 (ER-β1), and methylation accompanied by decreased expression for the caudal-related homeobox, transcription factor 2 (CDX2). While precursor lesions of these cancers, known as sessile serrated adenomas (SSA), were found to have decreased CDX2 expression, the status of ER-β1 expression in SSA is unknown. The aim of this study is to determine ER-β1 expression in SSA and its relation to CDX2 expression. METHODS: Sections of formalin fixed and paraffin embedded tissue from 62 consecutive cases of SSA were stained by immunohistochemistry for ER-β1 and CDX2. SSA with ER-β1 or CDX2 expression similar to that of a normal colon were scored as 0, while those with a loss of expression in <10% of SSA crypts as 1, 11-25% as 2, 26-50% as 3, 51-75% as 4, and CDX2 loss in >75% of the SSA crypts scored as 5. RESULTS: There is a significant correlation between a loss of CDX2 and the loss of ER-β1 scores in SSA (p<0.001). The downregulation of CDX2 was greater in SSA arising from the right colon compared to the left colon and rectum (p=0.012). Similarly, downregulation of ER-β1 was greater in SSA arising in the right colon compared to the left colon and rectum (p=0.014). CONCLUSIONS: Our findings show significant downregulation of both ER-β1 and CDX2 expression in SSA, especially in the right colon. These findings suggest that ER-β1 downregulation plays a significant role in the malignant progression of SSA.

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