Neutrophils contribute to ischemia/reperfusion injury in rat liver in vivo

Document Type

Journal Article

Publication Date

1-1-1990

Journal

FASEB Journal

Volume

4

Issue

15

DOI

10.1096/fasebj.4.15.2253850

Keywords

Anti-neutrophil antibody; Hepatic ischemia; Inflammation; Neutropenia; Peritonitis

Abstract

To determine the role of neutrophils in the pathogenesis of hepatic ischemia/reperfusion injury, livers from male Fischer rats were subjected to 45 min of no-flow ischemia followed by reperfusion for up to 24 h. Two phases of liver injury were identified, an initial phase during the first hour of reperfusion and a later progression phase with 80 ± 3% hepatocyte necrosis and an 80-fold increase of neutrophil infiltration in the liver after 24 h. Pretreatment with a monoclonal antibody against neutrophils, which caused consistent neutropenia, protected the liver from reperfusion injury as indicated by 28 ± 10% necrosis, and 84% reduction of hepatic neutrophil accumulation and a complete recovery of the hepatic ATP content. Our data suggest that the later progression phase of reperfusion injury after hepatic no-flow ischemia is mediated mainly by neutrophils.

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