Six mRNAs with different 5' ends are encoded by a single γ- glutamyltransferase gene in mouse

Document Type

Journal Article

Publication Date

1-1-1993

Journal

Proceedings of the National Academy of Sciences of the United States of America

Volume

90

Issue

13

DOI

10.1073/pnas.90.13.6179

Keywords

gene expression; kidney; promoter; transcription

Abstract

The 5' region of the mouse γ-glutamyltransferase (γGT; EC 2.3.2.2) gene has been cloned and analyzed. This analysis, combined with sequence information obtained from γGT cDNA clones, indicates that in mouse a single γGT gene codes for six different mRNAs that differ in their 5' sequences. Analysis of steady-state levels of γGT RNA reveals different expression patterns for these RNAs in different organs. The six different 5' sequences are widely separated within a 10-kb region and three of them show 75-86% identity with the three known rat γGT cDNAs. Although the heterogeneity of the 5' ends of γGT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.

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