Document Type
Journal Article
Publication Date
8-4-2021
Journal
Placenta
Volume
112
DOI
10.1016/j.placenta.2021.07.301
Keywords
Archived placenta; FFPE RNA; FFPE placenta; Formalin fixed paraffin embedded placenta; Pathology database; Placenta gene expression; Placenta pathology.
Abstract
The placenta is at the core of many pregnancy pathologies, but we have limited knowledge about placental function because of two key research barriers: 1) lack of guidelines for sample collection and pathologic diagnosis; and 2) limited tools are available for molecular analysis of stored placental samples. We aimed to create a searchable, population-based placental database of pathologic diagnoses, and to validate molecular methods for gene expression studies of matching formalin fixed paraffin embedded (FFPE) placental blocks. Our database has over 1000 pregnancies coded for clinical diagnosis with corresponding FFPE blocks that are available for gene expression studies. RNA harvested from FFPE tissues is of sufficient quality for downstream applications. We successfully used this pipeline to identify FFPE placenta from term and preterm pregnancies, and compared their gene expression. The establishment of this platform, which links clinicopathological data and molecular gene expression, will increase our understanding of obstetrical diseases.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
APA Citation
O'Reilly, J., Barak, S., & Penn, A. (2021). A new pipeline for clinico-pathological and molecular placental research utilizing FFPE tissues.. Placenta, 112 (). http://dx.doi.org/10.1016/j.placenta.2021.07.301
Peer Reviewed
1
Open Access
1
Comments
Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.