Lack of effects by σ ligands on neuropeptide Y-induced G-protein activation in rat hippocampus and cerebellum

Document Type

Journal Article

Publication Date

5-18-2001

Journal

Brain Research

Volume

901

Issue

1-2

DOI

10.1016/S0006-8993(01)02348-4

Keywords

[ S]GTPγS autoradiography 35; Cerebellum; GTPase; Hippocampus; Neuropeptide Y; Sigma receptor

Abstract

It has been suggested that neuropeptide Y (NPY) and sigma (σ) receptor ligands may share a putative NPY/σ receptor in rat brain. To study whether NPY and σ receptor ligands have an inverse agonism at this putative NPY/σ receptor, we measured their effects on G-protein activity in rat brain. Using [35S]GTPγS autoradiography, we found that NPY-induced G-protein activation exhibited a discrete distribution pattern in rat brain. G-protein activation in superficial cortical layers and hippocampal CA1-3 region was mainly attributed to Y1 and Y2 receptors, respectively. In the presence of 10 μM σ-receptor agonist BD737 or 10 μM σ-receptor antagonist haloperidol, the distribution and density of [35S]GTPγS binding stimulated by 10 nM NPY was not significantly altered. In rat cerebellar membranes, NPY stimulated high-affinity GTPase activity in a dose-related manner, with maximal effects of 29% increase over basal level seen at 500 nM. This NPY-elicited GTPase activity was not significantly affected by micromolar concentrations of the σ-receptor antagonists Dup734 or haloperidol. Since no significant effects by σ-receptor ligands on NPY-induced G-protein activation were observed, we did not see an inverse agonism of NPY and σ-receptor ligands at the putative NPY/σ receptor measured at the level of G-protein activation, suggesting that σ receptors and NPY receptors do not represent a common population in rat hippocampus and cerebellum. It is also suggested that G-protein activation is not a convergent point for the signal transduction mechanisms of NPY receptors and σ receptors. © 2001 Elsevier Science B.V. All rights reserved.

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