A concise guide to cDNA microarray analysis

Authors

P. Hegde, J. Craig Venter Institute
R. Qi, J. Craig Venter Institute
K. Abernathy, J. Craig Venter Institute
C. Gay, J. Craig Venter Institute
S. Dharap, J. Craig Venter Institute
R. Gaspard, J. Craig Venter Institute
J. E. Hughes, J. Craig Venter Institute
E. Snesrud, J. Craig Venter Institute
N. Lee, J. Craig Venter Institute
J. Quackenbush, J. Craig Venter Institute

Document Type

Journal Article

Publication Date

1-1-2000

Journal

BioTechniques

Volume

29

Issue

3

DOI

10.2144/00293bi01

Abstract

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

APA Citation

Hegde, P., Qi, R., Abernathy, K., Gay, C., Dharap, S., Gaspard, R., Hughes, J., Snesrud, E., Lee, N., & Quackenbush, J. (2000). A concise guide to cDNA microarray analysis. BioTechniques, 29 (3). http://dx.doi.org/10.2144/00293bi01