Identification of c-Myc responsive genes using rat cDNA microarray
c-Myc functions through direct activation or repression of transcription. Using cDNA microarray analysis, we have identified c-Myc-responsive genes by comparing gene expression profiles between c-myc null and c-myc wild-type rat fibroblast cells and between c-myc null and c-myc null cells reconstituted with c-myc. From a panel of 4400 cDNA elements, we found 198 genes responsive to c-myc when comparing wild-type or reconstituted cells with the null cells. The plurality of the named c-Myc-responsive genes that were up-regulated, including 30 ribosomal protein genes, are involved in macro-molecular synthesis and metabolism, suggesting a major role of c-Myc in the regulation of protein synthetic and metabolic pathways. When ectopically overexpressed, c-Myc induced a different and smaller set of c-Myc-responsive genes as compared with the physiologically expressed c-Myc condition. Thus, these results from expression profiling suggest a new primary function for c-Myc and raise the possibility that the physiological and transforming functions of c-myc may be separable.
Guo, Q., Malek, R., Kim, S., Chiao, C., He, M., Ruffy, M., Sanka, K., Lee, N., Dang, C., & Liu, E. (2000). Identification of c-Myc responsive genes using rat cDNA microarray. Cancer Research, 60 (21). Retrieved from https://hsrc.himmelfarb.gwu.edu/smhs_path_facpubs/1317