Document Type
Journal Article
Publication Date
12-29-2015
Journal
Oncotarget
Volume
6
Issue
42
Inclusive Pages
44927-40
DOI
10.18632/oncotarget.6743
Abstract
Male breast cancer comprises less than 1% of breast cancer diagnoses. Although estrogen exposure has been causally linked to the development of female breast cancers, the etiology of male breast cancer is unclear. Here, we show via fluorescence in situ hybridization (FISH) and droplet digital PCR (ddPCR) that the Y chromosome was clonally lost at a frequency of ~16% (5/31) in two independent cohorts of male breast cancer patients. We also show somatic loss of the Y chromosome gene TMSB4Y in a male breast tumor, confirming prior reports of loss at this locus in male breast cancers. To further understand the function of TMSB4Y, we created inducible cell lines of TMSB4Y in the female human breast epithelial cell line MCF-10A. Expression of TMSB4Y resulted in aberrant cellular morphology and reduced cell proliferation, with a corresponding reduction in the fraction of metaphase cells. We further show that TMSB4Y interacts directly with β-actin, the main component of the actin cytoskeleton and a cell cycle modulator. Taken together, our results suggest that clonal loss of the Y chromosome may contribute to male breast carcinogenesis, and that the TMSB4Y gene has tumor suppressor properties.
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
APA Citation
Wong, H., Wang, G., Croessmann, S., Zabransky, D., Aggarwal, A., Liu, M., & + 10 more (2015). TMSB4Y is a Candidate Tumor Suppressor on the Y Chromosome and is Deleted in Male Breast Cancer.. Oncotarget, 6 (42). http://dx.doi.org/10.18632/oncotarget.6743
Peer Reviewed
1
Open Access
1