Lipolysis of butter oil by an immobilized kid goat pregastric esterase
Document Type
Journal Article
Publication Date
1-1-2002
Journal
Journal of Food Science
Volume
67
Issue
8
DOI
10.1111/j.1365-2621.2002.tb08861.x
Keywords
Anhydrous milkfat; Hollow-fiber reactor; Immobilized lipase; Kid goat pregastric esterase; Lipolyzed butter oil
Abstract
A kid goat pregastric esterase immobilized in a continuous flow, hollow-fiber reactor was employed to hydrolyze anhydrous milkfat, producing lipolyzed butter oil. The effects of temperature and pH on the rate of lipolysis were monitored by titration of the effluent stream for total acidity. The overall rate of hydrolysis was fastest at 40°C and pH 6.0. The reaction rate was modeled by an expression of the general Michaelis-Menten form based on a ping pong bi bi mechanism. Nonlinear regression analyses indicated that the experimental data were consistent with the form of the ping pong bi bi mechanism that assumes that the rate-controlling step is acylation of the enzyme.
APA Citation
Hill, C., Ghannouchi, S., & Garcia, H. (2002). Lipolysis of butter oil by an immobilized kid goat pregastric esterase. Journal of Food Science, 67 (8). http://dx.doi.org/10.1111/j.1365-2621.2002.tb08861.x