Posterolateral intertransverse lumbar fusion in a mouse model: surgical anatomy and operative technique

Document Type

Journal Article

Publication Date

1-1-2007

Journal

Spine Journal

Volume

7

Issue

1

DOI

10.1016/j.spinee.2006.03.004

Keywords

Fusion model; Mouse; Transgenic technology

Abstract

Background context: Animal models are frequently used for studying the effect of bone graft substitutes or allogeneic materials on osterolateral lumbar fusion. Transgenic technology in the mouse provides a unique opportunity to further understand the biology of spine fusion. Purpose: To describe pertinent lumbar spine anatomy and formulate a surgical protocol for posterolateral fusion in the mouse model. Study design: Diagnostic model: development of an animal model for biologic evaluation of posterolateral spine fusion. Method: Ten mice were killed to study relevant lumbar spine anatomy and develop a protocol for lumbar spine fusion. The L4-L6 fusion protocol was validated in 46 mice for ease of exposure, preparation of the posterolateral fusion bed, introduction of bone inductive agents, and perioperative care. Results: Anatomy and surgical technique for posterolateral intertransverse lumbar fusion in the mouse model are described. A paraspinal approach allows exposure of the transverse processes, decortication, and graft placement at the L4-L6 intertransverse fusion site. Decortication alone did not result in fusion, whereas the use of bone graft resulted in satisfactory fusion rates. Perioperative morbidity and mortality rates were low. Conclusion: The mouse posterolateral lumbar spine fusion model is reproducible, inexpensive, and has low complication rates. Knowledge of the relevant anatomy and adherence to a well-defined surgical protocol provides a reliable and reproducible experimental spine fusion model. © 2007 Elsevier Inc. All rights reserved.

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