Effects of human serum and plasma on development of mouse embryos in culture media

Authors

B. Shirley, The University of Tulsa
J. W.E. Wortham, The University of Tulsa
J. Witmyer, The University of Tulsa
M. Condon-Mahony
G. Fort

Document Type

Journal Article

Publication Date

1-1-1985

Journal

Fertility and Sterility

Volume

43

Issue

1

DOI

10.1016/S0015-0282(16)48330-5

Abstract

Mouse embryos were cultured in Ham's F-10 medium (unsupplemented) and Hams F-10 medium supplemented with either 15% fetal cord serum, 15% fetal cord plasma, 15% maternal serum, or 15% maternal plasma. None of the blood supplements significantly increased the numbers of embryos that developed to blastocysts, and the last three of the blood supplements inhibited embryo development. The cause of the inhibition was not identified. Estradiol concentrations of blood samples used as media supplements were found not to correlate with inhibition of embryo development.

APA Citation

Shirley, B., Wortham, J., Witmyer, J., Condon-Mahony, M., & Fort, G. (1985). Effects of human serum and plasma on development of mouse embryos in culture media. Fertility and Sterility, 43 (1). http://dx.doi.org/10.1016/S0015-0282(16)48330-5