Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors
Journal of Obstetrics and Gynaecology Research
Complement system; Gene chip; Granulosa cells; Human follicular fluid; Mass spectrometry
Aims: In this study, we identify components of the complement system present in human follicular fluid that affect oocyte development and maturation. Material and Methods: Using bottom-up liquid chromatography/mass spectrometry/mass spectrometry, we identified complement factors as consistently present in human follicular fluid from 15 different subjects. Results: According to our gene-chip data, these complement factors are actively produced by granulosa cells. Conclusions: By applying the computational Ingenuity Pathway Analysis software and database we have identified complement pathways that play a role in oocyte maturation and follicular development. © 2012 Japan Society of Obstetrics and Gynecology.
Yoo, S., Bolbot, T., Koulova, A., Sneeringer, R., Humm, K., Dagon, Y., & Usheva, A. (2013). Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors. Journal of Obstetrics and Gynaecology Research, 39 (2). http://dx.doi.org/10.1111/j.1447-0756.2012.01985.x