Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors

Authors

Sang Wook Yoo, Departments of Medicine
Tetyana Bolbot, Departments of Medicine
Anna Koulova, Departments of Medicine
Rita Sneeringer, Harvard Medical School
Kathrin Humm, Harvard Medical School
Yossi Dagon, Departments of Medicine
Anny Usheva, Departments of Medicine

Document Type

Journal Article

Publication Date

2-1-2013

Journal

Journal of Obstetrics and Gynaecology Research

Volume

39

Issue

2

DOI

10.1111/j.1447-0756.2012.01985.x

Keywords

Complement system; Gene chip; Granulosa cells; Human follicular fluid; Mass spectrometry

Abstract

Aims: In this study, we identify components of the complement system present in human follicular fluid that affect oocyte development and maturation. Material and Methods: Using bottom-up liquid chromatography/mass spectrometry/mass spectrometry, we identified complement factors as consistently present in human follicular fluid from 15 different subjects. Results: According to our gene-chip data, these complement factors are actively produced by granulosa cells. Conclusions: By applying the computational Ingenuity Pathway Analysis software and database we have identified complement pathways that play a role in oocyte maturation and follicular development. © 2012 Japan Society of Obstetrics and Gynecology.

APA Citation

Yoo, S., Bolbot, T., Koulova, A., Sneeringer, R., Humm, K., Dagon, Y., & Usheva, A. (2013). Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors. Journal of Obstetrics and Gynaecology Research, 39 (2). http://dx.doi.org/10.1111/j.1447-0756.2012.01985.x