Document Type

Journal Article

Publication Date

1-1-2015

Journal

PLoS One

Volume

10

Issue

3

Inclusive Pages

Article Number e0119111

DOI

10.1371%2Fjournal.pone.0119111

Keywords

Astrocytes; Cell Line, Tumor; Cell Membrane; Cell Survival; Cold Temperature; Glioblastoma; Humans; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Neuroglia; Plasma Gases

Abstract

The atomic force microscope (AFM) is broadly used to study the morphology of cells. The morphological characteristics and differences of the cell membrane between normal human astrocytes and glial tumor cells are not well explored. Following treatment with cold atmospheric plasma, evaluation of the selective effect of plasma on cell viability of tumor cells is poorly understood and requires further evaluation. Using AFM we imaged morphology of glial cells before and after cold atmospheric plasma treatment. To look more closely at the effect of plasma on cell membrane, high resolution imaging was used. We report the differences between normal human astrocytes and human glioblastoma cells by considering the membrane surface details. Our data, obtained for the first time on these cells using atomic force microscopy, argue for an architectural feature on the cell membrane, i.e. brush layers, different in normal human astrocytes as compared to glioblastoma cells. The brush layer disappears from the cell membrane surface of normal E6/E7 cells and is maintained in the glioblastoma U87 cells after plasma treatment.

Comments

Reproduced with permission of PLoS ONE.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Peer Reviewed

1

Open Access

1

Download

Included in

Neurology Commons

Share

COinS