Document Type
Journal Article
Publication Date
2015
Journal
Scientific Reports
Volume
5
Inclusive Pages
18339
DOI
10.1038/srep18339
Abstract
To date, the significant anti-cancer capacity of cold atmospheric plasma (CAP) on dozens of cancer cell lines has been demonstrated in vitro and in mice models. Conventionally, CAP was directly applied to irradiate cancer cells or tumor tissue. Over past three years, the CAP irradiated media was also found to kill cancer cells as effectively as the direct CAP treatment. As a novel strategy, using the CAP stimulated (CAPs) media has become a promising anti-cancer tool. In this study, we demonstrated several principles to optimize the anti-cancer capacity of the CAPs media on glioblastoma cells and breast cancer cells. Specifically, using larger wells on a multi-well plate, smaller gaps between the plasma source and the media, and smaller media volume enabled us to obtain a stronger anti-cancer CAPs media composition without increasing the treatment time. Furthermore, cysteine was the main target of effective reactive species in the CAPs media. Glioblastoma cells were more resistant to the CAPs media than breast cancer cells. Glioblastoma cells consumed the effective reactive species faster than breast cancer cells did. In contrast to nitric oxide, hydrogen peroxide was more likely to be the effective reactive species.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
APA Citation
Yan, D., Talbot, A., Nourmohammadi, N., Cheng, X., Canady, J., Sherman, J., Keidar, M. (2015). Principles of using Cold Atmospheric Plasma Stimulated Media for Cancer Treatment. Scientific Reports, 5:18339. doi:10.1038/srep18339
Peer Reviewed
1
Open Access
1
Comments
Reproduced with permission of Macmillan Publishers Ltd. Scientific Reports.