Deep sequencing transcriptome analysis of murine wound healing: Effects of a multicomponent, multitarget natural product Therapy-Tr14

Document Type

Journal Article

Publication Date

8-17-2017

Journal

Frontiers in Molecular Biosciences

Volume

4

Issue

AUG

DOI

10.3389/fmolb.2017.00057

Keywords

Multicomponent; Multitarget; Natural products; RNA-seq; TGF-β1; Transcript profiling; Traumeel (Tr14); Wound healing

Abstract

© 2017 St. Laurent, Seilheimer, Tackett, Zhou, Shtokalo, Vyatkin, Ri, Toma, Jones and McCaffrey. Wound healing involves an orchestrated response that engages multiple processes, such as hemostasis, cellular migration, extracellular matrix synthesis, and in particular, inflammation. Using a murine model of cutaneous wound repair, the transcriptome was mapped from 12 h to 8 days post-injury, and in response to a multicomponent, multi-target natural product, Tr14. Using single-molecule RNA sequencing (RNA-seq), there were clear temporal changes in known transcripts related to wound healing pathways, and additional novel transcripts of both coding and non-coding genes. Tr14 treatment modulated > 100 transcripts related to key wound repair pathways, such as response to wounding, wound contraction, and cytokine response. The results provide the most precise and comprehensive characterization to date of the transcriptome's response to skin damage, repair, and multicomponent natural product therapy. By understanding the wound repair process, and the effects of natural products, it should be possible to intervene more effectively in diseases involving aberrant repair.

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