Elucidating the role of lipid rafts on g protein-coupled receptor function in the mouse kidney: An in vivo approach
Document Type
Journal Article
Publication Date
1-1-2021
Journal
Methods in Molecular Biology
Volume
2187
DOI
10.1007/978-1-0716-0814-2_10
Keywords
Kidney; Lipid raft; Methyl-β-cyclodextrin; Mini osmotic pump; Mouse; α-Cyclodextrin
Abstract
© 2021, Springer Science+Business Media, LLC, part of Springer Nature. Numerous G protein-coupled receptors (GPCRs) and GPCR-signaling molecules reside in lipid rafts and thus, are inherently regulated in these microdomains. However, the limitations of current methods to investigate lipid raft biology and GPCR activity in situ have hindered the complete understanding of the molecular underpinnings of GPCR trafficking and signaling, especially in the whole organism. This book chapter details an innovative in vivo approach to study the crucial role of lipid rafts on the workings of GPCRs in the mouse kidney. This protocol involves the use of a modified mini osmotic pump to deliver an agent that selectively disrupts the lipid raft in the kidney.
APA Citation
Asico, L., Rozyyev, S., Crusan, A., Jose, P., & Villar, V. (2021). Elucidating the role of lipid rafts on g protein-coupled receptor function in the mouse kidney: An in vivo approach. Methods in Molecular Biology, 2187 (). http://dx.doi.org/10.1007/978-1-0716-0814-2_10