Upregulation of renal D5 dopamine receptor ameliorates the hypertension in D3 dopamine receptor-deficient mice

Document Type

Journal Article

Publication Date

8-1-2013

Journal

Hypertension

Volume

62

Issue

2

DOI

10.1161/HYPERTENSIONAHA.113.01483

Keywords

Blood pressure; Dopamine; Mice, knockout; Receptors, dopamine

Abstract

D3 dopamine receptor (D3R)-deficient mice have renin-dependent hypertension associated with sodium retention, but the hypertension is mild. To determine whether any compensatory mechanisms in the kidney are involved in the regulation of blood pressure with disruption of Drd3, we measured the renal protein expression of all dopamine receptor subtypes (D1R, D2R, D4R, and D5R) in D 3R homozygous (D3-/-) and heterozygous (D 3+/-) knockout mice and their wild-type (D 3+/+) littermates. The renal immunohistochemistry and protein expression of D5R were increased (n=5/group) in D 3-/- mice; renal D4R protein expression was decreased, whereas renal protein expressions of D1R and D 2R were similar in both groups. Renal D5R protein expression was also increased in D3+/- (n=5/group) relative to D3+/+ mice, whereas D1R, D 2R, and D4R protein expressions were similar in D 3+/- and D3+/+ mice. The increase in renal D5R protein expression was abolished when D3-/- mice were fed a high-salt diet. Treatment with the D 1-like receptor antagonist, SCH23390, increased the blood pressure in anesthetized D3-/- but not D3+/+ mice (n=4/group), suggesting that the renal upregulation of D5R may have minimized the hypertension in D3-/- mice. The renal D5R protein upregulation was not caused by increased transcription because renal mRNA expression of D5R was similar in D 3-/- and D3+/+ mice. Our findings suggest that the renal upregulation of D5R may have minimized the hypertension that developed in D3-/- mice. © 2013 American Heart Association, Inc.

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