Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis.

Authors

Sharlaa Badal-Faesen
Cynthia Firnhaber
Michelle A Kendall
Xingye Wu
Beatriz Grinsztejn
Rodrigo Otavio da Silva Escada
Michel Fernandez
Evelyn Hogg
Ian Sanne
Pamela Johnson
David Alland
Gerald H Mazurek
Debra A Benator, George Washington UniversityFollow
Anne F Luetkemeyer

Document Type

Journal Article

Publication Date

8-7-2017

Journal

Journal of Clinical Medicine

Volume

6

Issue

8

DOI

10.3390/jcm6080078

Abstract

As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8)

Comments

Reproduced with permission of MDPI AG. Clinical Medicine

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

APA Citation

Badal-Faesen, S., Firnhaber, C., Kendall, M., Wu, X., Grinsztejn, B., Escada, R., Fernandez, M., Hogg, E., Sanne, I., Johnson, P., Alland, D., Mazurek, G., Benator, D., & Luetkemeyer, A. (2017). Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis.. Journal of Clinical Medicine, 6 (8). http://dx.doi.org/10.3390/jcm6080078

Peer Reviewed

1

Open Access

1