Document Type
Journal Article
Publication Date
9-2012
Journal
Brain and Behavior
Volume
Volume 2, Issue 5
Inclusive Pages
563-575
Abstract
Galectins are pleiotropic carbohydrate-binding lectins involved in inflammation, growth/differentiation, and tissue remodeling. The functional role of galectins in amyotrophic lateral sclerosis (ALS) is unknown. Expression studies revealed increases in galectin-1 mRNA and protein in spinal cords from SOD1 G93A mice, and in galectin-3 and -9 mRNAs and proteins in spinal cords of both SOD1G93A mice and sporadic ALS patients. As the increase in galectin-3 appeared in early presymptomatic stages and increased progressively through to end stage of disease in the mouse, it was selected for additional study, where it was found to be mainly expressed by microglia. Galectin-3 antagonists are not selective and do not readily cross the blood–brain barrier; therefore, we generated SOD1 G93A/Gal-3 −/− transgenic mice to evaluate galectin-3 deletion in a widely used mouse model of ALS. Disease progression, neurological symptoms, survival, and inflammation were assessed to determine the effect of galectin-3 deletion on the SOD1 G93A disease phenotype. Galectin-3 deletion did not change disease onset, but resulted in more rapid progression through functionally defined disease stages, more severely impaired neurological symptoms at all stages of disease, and expiration, on average, 25 days earlier than SOD1 G93A/Gal-3 +/+ cohorts. In addition, microglial staining, as well as TNF-α, and oxidative injury were increased in SOD1 G93A/Gal-3 −/− mice compared with SOD1 G93A/Gal-3 +/+ cohorts. These data support an important functional role for microglial galectin-3 in neuroinflammation during chronic neurodegenerative disease. We suggest that elevations in galectin-3 by microglia as disease progresses may represent a protective, anti-inflammatory innate immune response to chronic motor neuron degeneration.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License
APA Citation
Lerman, B. J., Hoffman, E. P., Sutherland, M. L., Bouri, K., Hsu, D. K., Liu, F.-T., Rothstein, J. D., Knoblach, S. M. (2012). Deletion of galectin-3 exacerbates microglial activation and accelerates disease progression and demise in a SOD1 G93A mouse model of amyotrophic lateral sclerosis. Brain and Behavior, 2: 563–575
Peer Reviewed
1
Open Access
1
Comments
Reproduced with permission of Wiley - Brain and Behavior.