Document Type
Journal Article
Publication Date
8-13-2014
Journal
PLoS ONE
Volume
Volume 9, Issue 8
Inclusive Pages
Article number e104070
DOI
10.1371/journal.pone.0104070
Abstract
Ciliary accumulation of signaling proteins must result from a rate of ciliary entry that exceeds ciliary exit, but approaches for distinguishing ciliary entry vs. exit are lacking. Using a photoconvertible fluorescent protein tag, we establish an assay that allows a separate but simultaneous examination of ciliary entry and exit of the Hedgehog signaling protein Smoothened in individual cells. We show that KAAD-cyclopamine selectively blocks entry, whereas ciliobrevin interferes initially with exit and eventually with both entry and exit of ciliary Smoothened. Our study provides an approach to understanding regulation of ciliary entry vs. exit of Hedgehog signaling components as well as other ciliary proteins.
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
APA Citation
Kim, J., Hsia, E.Y.C., Kim, J., Sever, N., Beachy, P.A. et al. (2014). Simultaneous measurement of smoothened entry into and exit from the primary cilium. PLoS ONE, 9(8):e104070.
Peer Reviewed
1
Open Access
1
Comments
Reproduced with permission of PLoS ONE.