Title

The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.

Document Type

Journal Article

Publication Date

1-1-2022

Journal

Methods in molecular biology (Clifton, N.J.)

Volume

2374

DOI

10.1007/978-1-0716-1701-4_8

Keywords

Cilia; Cultured cells; Hedgehog (Hh) signaling; Immunofluorescence; Smoothened (Smo).

Abstract

The primary cilium is a microtubule-based organelle that projects from the surface of vertebrate cells. Defects in the biogenesis of or transport through primary cilia affect Hedgehog signaling, and many Hedgehog pathway components traffic through or accumulate in cilia. This protocol provides methods for immunofluorescence staining of cilia-accumulated Hh pathway components, such as Smoothened, in cultured NIH 3T3 cells.

Peer Reviewed

1

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