The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.
Methods in molecular biology (Clifton, N.J.)
Cilia; Cultured cells; Hedgehog (Hh) signaling; Immunofluorescence; Smoothened (Smo).
The primary cilium is a microtubule-based organelle that projects from the surface of vertebrate cells. Defects in the biogenesis of or transport through primary cilia affect Hedgehog signaling, and many Hedgehog pathway components traffic through or accumulate in cilia. This protocol provides methods for immunofluorescence staining of cilia-accumulated Hh pathway components, such as Smoothened, in cultured NIH 3T3 cells.
Yang, S., & Zheng, X. (2022). The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.. Methods in molecular biology (Clifton, N.J.), 2374 (). http://dx.doi.org/10.1007/978-1-0716-1701-4_8