The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.
Document Type
Journal Article
Publication Date
1-1-2022
Journal
Methods in molecular biology (Clifton, N.J.)
Volume
2374
DOI
10.1007/978-1-0716-1701-4_8
Keywords
Cilia; Cultured cells; Hedgehog (Hh) signaling; Immunofluorescence; Smoothened (Smo).
Abstract
The primary cilium is a microtubule-based organelle that projects from the surface of vertebrate cells. Defects in the biogenesis of or transport through primary cilia affect Hedgehog signaling, and many Hedgehog pathway components traffic through or accumulate in cilia. This protocol provides methods for immunofluorescence staining of cilia-accumulated Hh pathway components, such as Smoothened, in cultured NIH 3T3 cells.
APA Citation
Yang, S., & Zheng, X. (2022). The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.. Methods in molecular biology (Clifton, N.J.), 2374 (). http://dx.doi.org/10.1007/978-1-0716-1701-4_8
Peer Reviewed
1
