Escherichia coli ST117: exploring the zoonotic hypothesis

A B. Saidenberg, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
S M. Edslev, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
S Hallstrøm, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
A Rasmussen, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
D E. Park, Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
M Aziz, Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
B Dos Santos Queiroz, School of Veterinary Medicine and Animal Science, São Paulo, Brazil.
A A. Baptista, Preventive Veterinary Medicine Department, State University of Londrina, Parana, Brazil.
F Barbosa, School of Veterinary Medicine and Animal Science, São Paulo, Brazil.
V G. Rocha, School of Veterinary Medicine and Animal Science, São Paulo, Brazil.
Arnoud H. van Vliet, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom.
A Dalsgaard, Section for Food Safety and Zoonoses, Institute for Veterinary and Companion Animal Science, Københavns Universitet, Copenhagen, Denmark.
L B. Price, Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
T Knöbl, School of Veterinary Medicine and Animal Science, São Paulo, Brazil.
M Stegger, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.

Document Type

Journal Article

Publication Date

9-5-2024

Journal

Microbiology spectrum

DOI

10.1128/spectrum.00466-24

Keywords

Escherichia coli; ExPEC; FZEC; ST117; poultry

Abstract

UNLABELLED: Extraintestinal pathogenic Escherichia coli (ExPEC) can lead to severe infections, with additional risks of increasing antimicrobial resistance rates. Genotypic similarities between ExPEC and avian pathogenic E. coli (APEC) support a possible role for a poultry meat reservoir in human disease. Some genomic studies have been done on the ST117 lineage which contaminates poultry meat, carries multidrug resistance, can be found in the human intestinal microbiota, and causes human extraintestinal disease. This study analyzed the genomes of 61 E. coli from Brazilian poultry outbreaks focusing on ST117, to further define its possible zoonotic characteristics by genotypic and phylogenomic analyses, along with 1,699 worldwide ST117 isolates originating from human, animal, and environment sources. A predominance of ST117 was detected in the Brazilian isolates (n = 20/61) frequently carrying resistance to critical antibiotics (>86%) linked to IncFII, IncI1, or IncX4 replicons. High similarities were found between IncX4 from Brazilian outbreaks and those from E. coli recovered from imported Brazilian poultry meat and human clinical cases. The ST117 phylogeny showed non-specificity according to host and continent and an AMR index score indicated the highest resistance in Asia and South America, with the latter statistically more resistant and overrepresented with resistance to extended-spectrum beta-lactamases (ESBL). Most ST117 human isolates were predicted to have a poultry origin (93%, 138/148). In conclusion, poultry is a likely source for zoonotic ExPEC strains, particularly the ST117 lineage which can also serve as a reservoir for resistance determinants against critical antibiotics encoded on highly transmissible plasmids. IMPORTANCE: Certain extraintestinal pathogenic Escherichia coli (ExPEC) are particularly important as they affect humans and animals. Lineages, such as ST117, are predominant in poultry and frequent carriers of antibiotic resistance, presenting a risk to humans handling or ingesting poultry products. We analyzed ExPEC isolates causing outbreaks in Brazilian poultry, focusing on the ST117 as the most detected lineage. Genomic comparisons with international isolates from humans and animals were performed describing the potential zoonotic profile. The Brazilian ST117 isolates carried resistance determinants against critical antibiotics, mainly on plasmids, in some cases identical to those carried by international isolates. South American ST117 isolates from all sources generally exhibit more resistance, including to critical antibiotics, and worldwide, the vast majority of human isolates belonging to this lineage have a predicted poultry origin. As the world's largest poultry exporter, Brazil has an important role in developing strategies to prevent the dissemination of multidrug-resistant zoonotic ExPEC strains.

Department

Environmental and Occupational Health

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