Topical Nanoencapsulated Cannabidiol Cream as an Innovative Strategy Combatting Ultraviolet A-Induced Nuclear and Mitochondrial DNA Injury: A Pilot Randomized Clinical Study
Document Type
Journal Article
Publication Date
7-16-2024
Journal
Journal of the American Academy of Dermatology
DOI
10.1016/j.jaad.2024.06.088
Keywords
8-oxo-guanine; CBD; Photoprotection; ROS; UVA; Ultraviolet-A; cannabidiol; cannabinoid; common deletion; mitochondrial DNA; mitochondrial mutation; photoaging; photodamage; reactive oxygen species
Abstract
BACKGROUND: Ultraviolet-A radiation (UVA) contributes to photoaging/photocarcinogenesis by generating inflammation and oxidative damage. Current photoprotective strategies are limited by availability/utilization of UVA filters, highlighting an unmet need. Cannabidiol (CBD), having anti-inflammatory/antioxidant properties via regulation of NFR-2, HMOX1, and PPAR-y, could potentially mitigate damage from UVA exposure. OBJECTIVE/METHODS: Prospective, single-center, pilot clinical trial (NCT05279495). Nineteen participants applied nano-CBD (nCBD) or vehicle (VC) cream to randomized, blinded buttock sites twice-daily for 14-days, then treated sites were irradiated with ≤3x UVA minimal erythema dose. After 24-hours, punch biopsies were obtained for histology, immunohistochemistry, real-time PCR. RESULTS: At 24-hours, 21% of participants had less observed erythema on CBD-treated skin than VC skin. Histologically, nCBD-treated skin had reduced UVA-induced epidermal hyperplasia than VC (p=0.01). Immunohistochemistry detected reduced cytoplasmic/nuclear 8-oxo-guanine glycosylase 1 staining in nCBD-treated skin compared to VC (p<0.01). Quantitative mtDNA PCR demonstrated UVA-induced deletion of ND4 (proxy:4977bp deletion; p=0.003) and ND1 (proxy:3895bp deletion; p=0.002) were significantly reduced by in vivo nCBD treatment compared to VC. LIMITATIONS: Sample size. CONCLUSION: Topically applied nCBD cream reduced UVA-induced formation of a frequent mutagenic nuclear DNA base lesion and protected against mtDNA mutations associated with UVA-induced skin aging. This trial is the first to identify UV-protective capacity of CBD-containing topicals in humans.
APA Citation
McCormick, Erika; Han, Haowei; Abdel Azim, Sara; Whiting, Cleo; Bhamidipati, Nitish; Kiss, Alexi; Efimova, Tatiana; Berman, Brian; and Friedman, Adam, "Topical Nanoencapsulated Cannabidiol Cream as an Innovative Strategy Combatting Ultraviolet A-Induced Nuclear and Mitochondrial DNA Injury: A Pilot Randomized Clinical Study" (2024). GW Authored Works. Paper 5271.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/5271
Department
Dermatology