Probiotic KUB-AC5 decreases urothelial cell invasion and enhances macrophage killing of uropathogenic study

Arishabhas Tantibhadrasapa, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Songbo Li, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Songphon Buddhasiri, Research Center for Veterinary Biosciences and Veterinary Public Health, Chiang Mai University, Chiang Mai, Thailand.
Chutikarn Sukjoi, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Panupon Mongkolkarvin, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Pattarapon Boonpan, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Somsakul Pop Wongpalee, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Prasobsook Paenkaew, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Sawannee Sutheeworapong, Pilot Plant Development and Training Institute (PDTI), King Mongkut's University of Technology Thonburi (KMUTT), Bangkok, Thailand.
Massalin Nakphaichit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand.
Sunee Nitisinprasert, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand.
Michael H. Hsieh, Department of Urology, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States.
Parameth Thiennimitr, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Document Type

Journal Article

Publication Date

1-1-2024

Journal

Frontiers in cellular and infection microbiology

Volume

14

DOI

10.3389/fcimb.2024.1401462

Keywords

Limosilactobacillus reuteri; immune modulation; immunobiotic; probiotic; urinary tract infection (UTI); uropathogenic E. coli (UPEC)

Abstract

INTRODUCTION: Bacterial urinary tract infections (UTI) are among the most common infectious diseases worldwide. The rise of multidrug-resistant (MDR) uropathogenic (UPEC) UTI cases is a significant threat to healthcare systems. Several probiotic bacteria have been proposed as an alternative to combat MDR UTI. Lactic acid bacteria in the genus are some of the most studied and used probiotics. However, strain-specific effects play a critical role in probiotic properties. KUB-AC5 (AC5), isolated from the chicken gut, confers antimicrobial and immunobiotic effects against some human pathogens. However, the antibacterial and immune modulatory effects of AC5 on UPEC have never been explored. METHODS: Here, we investigated both the direct and indirect effects of AC5 against UPEC isolates (UTI89, CFT073, and clinical MDR UPEC AT31) . Using a spot-on lawn, agar-well diffusion, and competitive growth assays, we found that viable AC5 cells and cell-free components of this probiotic significantly reduced the UPEC growth of all strains tested. The human bladder epithelial cell line UM-UC-3 was used to assess the adhesion and pathogen-attachment inhibition properties of AC5 on UPEC. RESULTS AND DISCUSSION: Our data showed that AC5 can attach to UM-UC-3 and decrease UPEC attachment in a dose-dependent manner. Pretreatment of UPEC-infected murine macrophage RAW264.7 cells with viable AC5 (multiplicity of infection, MOI = 1) for 24 hours enhanced macrophage-killing activity and increased proinflammatory (, , and ) and anti-inflammatory () gene expression. These findings indicate the gut-derived AC5 probiotic could be a potential urogenital probiotic against MDR UTI.

Department

Urology

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