Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia
Document Type
Journal Article
Publication Date
4-1-2024
Journal
Clinical immunology (Orlando, Fla.)
Volume
261
DOI
10.1016/j.clim.2024.109942
Keywords
Mitogen; PHA; PIDTC; SCID; Severe combined immunodeficiency; T-cell lymphopenia; T-cell proliferation
Abstract
Severe combined immunodeficiency (SCID) is characterized by a severe deficiency in T cell numbers. We analyzed data collected (n = 307) for PHA-based T cell proliferation from the PIDTC SCID protocol 6901, using either a radioactive or flow cytometry method. In comparing the two groups, a smaller number of the patients tested by flow cytometry had <10% of the lower limit of normal proliferation as compared to the radioactive method (p = 0.02). Further, in patients with CD3+ T cell counts between 51 and 300 cells/μL, there was a higher proliferative response with the PHA flow assay compared to the H-T assay (p < 0.0001), suggesting that the method of analysis influences the resolution and interpretation of PHA results. Importantly, we observed many SCID patients with profound T cell lymphopenia having normal T cell proliferation when assessed by flow cytometry. We recommend this test be considered only as supportive in the diagnosis of typical SCID.
APA Citation
Abraham, Roshini S.; Basu, Amrita; Heimall, Jennifer R.; Dunn, Elizabeth; Yip, Alison; Kapadia, Malika; Kapoor, Neena; Satter, Lisa Forbes; Buckley, Rebecca; O'Reilly, Richard; Cuvelier, Geoffrey D.; Chandra, Sharat; Bednarski, Jeffrey; Chaudhury, Sonali; Moore, Theodore B.; Haines, Hilary; Dávila Saldaña, Blachy J.; Chellapandian, Deepakbabu; Rayes, Ahmad; Chen, Karin; Caywood, Emi; Chandrakasan, Shanmuganathan; Lugt, Mark Thomas; Ebens, Christen; Teira, Pierre; Shereck, Evan; Miller, Holly; Aquino, Victor; Eissa, Hesham; Yu, Lolie C.; Gillio, Alfred; and Madden, Lisa, "Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia" (2024). GW Authored Works. Paper 4819.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/4819
Department
Pediatrics