The Cultured T Model of HIV Latency

Authors

Alberto Bosque, Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC, USA. abosque@email.gwu.edu.

Document Type

Journal Article

Publication Date

1-1-2022

Journal

Methods in molecular biology (Clifton, N.J.)

Volume

2407

DOI

10.1007/978-1-0716-1871-4_4

Keywords

Central Memory CD4T cells; Latency; Latency-reversal; Replication-competent HIV; Reservoirs

Abstract

Models to study HIV latency have improved our understanding of the mechanisms involved in this process and have helped in the discovery and development of therapeutic strategies to eradicate HIV. Primary cell models are based on the in vitro generation of latently infected cells using CD4T cells isolated from blood, lymph nodes or other lymphoid organs. In this chapter, we describe the generation of HIV latently infected memory CD4T cells using blood naïve CD4T cells from peripheral blood with a phenotype resembling that of central memory CD4T cells. This model can be used to investigate the mechanisms involved in latency as well to develop strategies to target it.

APA Citation

Bosque, Alberto, "The Cultured T Model of HIV Latency" (2022). GW Authored Works. Paper 471.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/471

Department

Microbiology, Immunology, and Tropical Medicine