Association of oral microbiome with oral human papillomavirus infection: a population study of the National Health and Nutrition Examination Survey, 2009-2012

Xinyi Feng, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Eshan U. Patel, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jodie L. White, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Shilan Li, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Xianming Zhu, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ni Zhao, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Jianxin Shi, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Daniel Park, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
Cindy M. Liu, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
Rupert Kaul, Departments of Medicine and Immunology, University of Toronto, Toronto, ON, Canada.
Jessica L. Prodger, Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada.
Thomas C. Quinn, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
M Kate Grabowski, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Aaron A. Tobian, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Document Type

Journal Article

Publication Date

1-5-2024

Journal

The Journal of infectious diseases

DOI

10.1093/infdis/jiae004

Keywords

HPV; National Health and Nutrition Examination Survey; human papillomavirus; oral microbiome

Abstract

BACKGROUND: Oral human papillomavirus(HPV) infection and the oral microbiome are associated with oropharyngeal cancer. However, population-based data on the association of oral microbiome with oral HPV infection are limited. METHOD: We performed a cross-sectional analysis of 5,496 participants aged 20-59 in National Health and Nutrition Examination Surveys(NHANES):2009-2012. The association between either oral microbiome alpha diversity or beta diversity and oral HPV infection was assessed using multivariable logistic regression or principal coordinate analyses(PCoA) and multivariate analysis of variance(PERMANOVA). RESULTS: For alpha diversity, we found a lower number of observed Amplicon sequence variants(ASVs) (adjusted odds ratio[aOR] = 0.996; 95%CI = 0.992-0.999) and reduced Faith's Phylogenetic Diversity(aOR = 0.95; 95%CI = 0.90-0.99) associated with high-risk oral HPV infection in the overall population. This trend was observed in males for both high-risk and any oral HPV infection. Beta diversity showed differentiation of oral microbiome community by high-risk oral HPV infection as measured by Bray-Curtis dissimilarity (R2 = 0.054%; P = .029) and unweighted UniFrac distance (R2 = 0.046%; P = .045) among the overall population, and associations were driven by males. CONCLUSIONS: Both oral microbiome alpha diversity(within-sample richness and phylogenetic diversity) and beta diversity(heterogeneous dispersion of oral microbiome community) are associated with HPV infection. Longitudinal studies are needed to characterize the role of the microbiome in the natural history of oral HPV infection.

Department

Environmental and Occupational Health

Link to Full Text

Share

COinS