Review of flow cytometry as a tool for cell and gene therapy

Authors

Christopher A. Lazarski, Program for Cell Enhancement and Technology for Immunotherapy, Center for Cancer and Immunology Research, Children's National Hospital, Washington, DC, USA; The George Washington University, Washington, DC, USA. Electronic address: CLazarsk@childrensnational.org.
Patrick J. Hanley, Program for Cell Enhancement and Technology for Immunotherapy, Center for Cancer and Immunology Research, Children's National Hospital, Washington, DC, USA; The George Washington University, Washington, DC, USA. Electronic address: PHanley@childrensnational.org.

Document Type

Journal Article

Publication Date

11-7-2023

Journal

Cytotherapy

DOI

10.1016/j.jcyt.2023.10.005

Keywords

cellular therapy; flow cytometry; quality control

Abstract

Quality control testing and analytics are critical for the development and manufacture of cell and gene therapies, and flow cytometry is a key quality control and analytical assay that is used extensively. However, the technical scope of characterization assays and safety assays must keep apace as the breadth of cell therapy products continues to expand beyond hematopoietic stem cell products into producing novel adoptive immune therapies and gene therapy products. Flow cytometry services are uniquely positioned to support the evolving needs of cell therapy facilities, as access to flow cytometers, new antibody clones and improved fluorochrome reagents becomes more egalitarian. This report will outline the features, logistics, limitations and the current state of flow cytometry within the context of cellular therapy.

APA Citation

Lazarski, Christopher A. and Hanley, Patrick J., "Review of flow cytometry as a tool for cell and gene therapy" (2023). GW Authored Works. Paper 3832.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/3832

Department

Pediatrics