MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells

Authors

Pedro Felipe Gardeazabal Rodriguez, Department of Anatomy and Cell Biology, George Washington University, School of Medicine and Health Sciences, Washington, DC, USA.
Yigal Lilach, Nanofabrication and Imaging Center, George Washington University, Washington, DC, USA.
Abhijit Ambegaonkar, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institutes of Health, Rockville, MD, USA.
Teresa Vitali, Department of Anatomy and Cell Biology, George Washington University, School of Medicine and Health Sciences, Washington, DC, USA.
Haani Jafri, Department of Neuroscience, Thomas Jefferson University, Philadelphia, PA, USA.
Hae Won Sohn, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institutes of Health, Rockville, MD, USA.
Matthew Dalva, Department of Neuroscience, Thomas Jefferson University, Philadelphia, PA, USA.
Susan Pierce, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institutes of Health, Rockville, MD, USA.
Inhee Chung, Department of Anatomy and Cell Biology, George Washington University, School of Medicine and Health Sciences, Washington, DC, USA. inheec@gwu.edu.

Document Type

Journal Article

Publication Date

10-12-2023

Journal

Communications biology

Volume

6

Issue

1

DOI

10.1038/s42003-023-05380-2

Abstract

Mapping 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such as 3D-structured illumination microscopy (3D-SIM) can achieve twice the axial ( ~ 300 nm) and lateral ( ~ 100 nm) resolution of widefield microscopy in real time in live cells. However, twice-resolution enhancement cannot sufficiently visualize nanoscale fine structures of the plasma membrane. Axial interferometry methods including fluorescence light interference contrast microscopy and its derivatives (e.g., scanning angle interference microscopy) can determine nanoscale axial locations of proteins on and near the plasma membrane. Thus, by combining super-resolution lateral imaging of 2D-SIM with axial interferometry, we developed multi-angle-crossing structured illumination microscopy (MAxSIM) to generate multiple incident angles by fast, optoelectronic creation of diffraction patterns. Axial localization accuracy can be enhanced by placing cells on a bottom glass substrate, locating a custom height-controlled mirror (HCM) at a fixed axial position above the glass substrate, and optimizing the height reconstruction algorithm for noisy experimental data. The HCM also enables imaging of both the apical and basal surfaces of a cell. MAxSIM with HCM offers high-fidelity nanoscale 3D topological mapping of cell plasma membranes with near-real-time ( ~ 0.5 Hz) imaging of live cells and 3D single-molecule tracking.

APA Citation

Gardeazabal Rodriguez, Pedro Felipe; Lilach, Yigal; Ambegaonkar, Abhijit; Vitali, Teresa; Jafri, Haani; Sohn, Hae Won; Dalva, Matthew; Pierce, Susan; and Chung, Inhee, "MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells" (2023). GW Authored Works. Paper 3612.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/3612

Department

Anatomy and Regenerative Biology