Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans
Cell Isolation; Clinical Protocol; Immunology
The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022)..
Scholte, Larissa L.; Leggat, David J.; Cohen, Kristen W.; Hoeweler, Lara; Erwin, Guacyara C.; Rahaman, Farhard; Lombardo, Angela; Philiponis, Vincent; Laufer, Dagna S.; Siefers, Heather; Ruppel, Alexis M.; Brand, Joshua; Maenza, Janine; Bronson, Rhi; Prabhakaran, Madhu; Jean-Baptiste, Jalen; Kolokythas, Orpheus; Desrosiers, Aimee A.; Thoreson, Caroline K.; Heit, Antje; Khati, Nadia J.; Malkin, Elissa; McElrath, M Juliana; McDermott, Adrian B.; Schief, William R.; Diemert, David; and Bethony, Jeffrey M., "Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans" (2023). GW Authored Works. Paper 3371.
Microbiology, Immunology, and Tropical Medicine