Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans

Larissa L. Scholte, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA. Electronic address: larissascholte@gwu.edu.
David J. Leggat, The US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Kristen W. Cohen, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
Lara Hoeweler, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
Guacyara C. Erwin, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
Farhard Rahaman, IAVI, New York, NY 10004, USA.
Angela Lombardo, IAVI, New York, NY 10004, USA.
Vincent Philiponis, IAVI, New York, NY 10004, USA.
Dagna S. Laufer, IAVI, New York, NY 10004, USA.
Heather Siefers, IAVI, New York, NY 10004, USA.
Alexis M. Ruppel, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Joshua Brand, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Janine Maenza, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA.
Rhi Bronson, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Madhu Prabhakaran, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Jalen Jean-Baptiste, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Orpheus Kolokythas, Department of Radiology, University of Washington, Seattle, WA 98195, USA.
Aimee A. Desrosiers, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
Caroline K. Thoreson, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
Antje Heit, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
Nadia J. Khati, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
Elissa Malkin, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
M Juliana McElrath, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA.
Adrian B. McDermott, Vaccines Research and Development, Sanofi, Swiftwater, PA 183709, USA.
William R. Schief, IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Center for HIV/AIDS Vaccine Development, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA; The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02139, USA.
David Diemert, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
Jeffrey M. Bethony, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.

Document Type

Journal Article

Publication Date

9-20-2023

Journal

STAR protocols

Volume

4

Issue

4

DOI

10.1016/j.xpro.2023.102576

Keywords

Cell Isolation; Clinical Protocol; Immunology

Abstract

The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022)..

Department

Microbiology, Immunology, and Tropical Medicine

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