Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans

Authors

• Larissa L. Scholte, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA. Electronic address: larissascholte@gwu.edu.
• David J. Leggat, The US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
• Kristen W. Cohen, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
• Lara Hoeweler, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• Guacyara C. Erwin, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• Farhard Rahaman, IAVI, New York, NY 10004, USA.
• Angela Lombardo, IAVI, New York, NY 10004, USA.
• Vincent Philiponis, IAVI, New York, NY 10004, USA.
• Dagna S. Laufer, IAVI, New York, NY 10004, USA.
• Heather Siefers, IAVI, New York, NY 10004, USA.
• Alexis M. Ruppel, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
• Joshua Brand, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
• Janine Maenza, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA.
• Rhi Bronson, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
• Madhu Prabhakaran, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
• Jalen Jean-Baptiste, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
• Orpheus Kolokythas, Department of Radiology, University of Washington, Seattle, WA 98195, USA.
• Aimee A. Desrosiers, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• Caroline K. Thoreson, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• Antje Heit, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
• Nadia J. Khati, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• Elissa Malkin, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• M Juliana McElrath, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA.
• Adrian B. McDermott, Vaccines Research and Development, Sanofi, Swiftwater, PA 183709, USA.
• William R. Schief, IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Center for HIV/AIDS Vaccine Development, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA; The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02139, USA.
• David Diemert, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
• Jeffrey M. Bethony, Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.

Document Type

Journal Article

Publication Date

9-20-2023

Journal

STAR protocols

Volume

4

Issue

4

DOI

10.1016/j.xpro.2023.102576

Keywords

Cell Isolation; Clinical Protocol; Immunology

Abstract

The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022)..

APA Citation

Scholte, Larissa L.; Leggat, David J.; Cohen, Kristen W.; Hoeweler, Lara; Erwin, Guacyara C.; Rahaman, Farhard; Lombardo, Angela; Philiponis, Vincent; Laufer, Dagna S.; Siefers, Heather; Ruppel, Alexis M.; Brand, Joshua; Maenza, Janine; Bronson, Rhi; Prabhakaran, Madhu; Jean-Baptiste, Jalen; Kolokythas, Orpheus; Desrosiers, Aimee A.; Thoreson, Caroline K.; Heit, Antje; Khati, Nadia J.; Malkin, Elissa; McElrath, M Juliana; McDermott, Adrian B.; Schief, William R.; Diemert, David; and Bethony, Jeffrey M., "Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans" (2023). GW Authored Works. Paper 3371.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/3371

Department

Microbiology, Immunology, and Tropical Medicine