Poor Sensitivity of the MALDI Biotyper MBT Subtyping Module for Detection of Carbapenemase (KPC) in Species
Antibiotics (Basel, Switzerland)
CRE; KPC; Klebsiella; MALDI-ToF mass spectrometry; carbapenem resistance
Rapid detection of carbapenemase (KPC) in the species is desirable. The MALDI Biotyper MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical isolates, with whole genome sequences used to assess for and . For the isolates identified as KPC positive by sequencing, the overall sensitivity of the MALDI-ToF MS subtyping module was 239/574 (42%) with 100% specificity. For the isolates harboring , the subtyping module showed a sensitivity of 97% (239/246) and a specificity of 100%. The subtyping module had poor sensitivity for the detection of -positive isolates, albeit exhibiting excellent specificity. The poor sensitivity was a result of being present in only 43% of the -positive isolates.
Cuello, Luz; Alvarez Otero, Judith; Greenwood-Quaintance, Kerryl E.; Chen, Liang; Hanson, Blake; Reyes, Jinnethe; Komarow, Lauren; Ge, Lizhao; Lancaster, Zane D.; Gordy, Garrett G.; Schuetz, Audrey N.; and Patel, Robin, "Poor Sensitivity of the MALDI Biotyper MBT Subtyping Module for Detection of Carbapenemase (KPC) in Species" (2023). GW Authored Works. Paper 3369.
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