Poor Sensitivity of the MALDI Biotyper MBT Subtyping Module for Detection of Carbapenemase (KPC) in Species

Authors

Luz Cuello, Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
Judith Alvarez Otero, Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
Kerryl E. Greenwood-Quaintance, Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
Liang Chen, Hackensack Meridian Health Center for Discovery and Innovation, Nutley, NJ 07110, USA.
Blake Hanson, Department of Epidemiology, Human Genetics & Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
Jinnethe Reyes, Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá 110121, Colombia.
Lauren Komarow, The Biostatistics Center, The George Washington University, Rockville, MD 20852, USA.
Lizhao Ge, The Biostatistics Center, The George Washington University, Rockville, MD 20852, USA.
Zane D. Lancaster, Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Garrett G. Gordy, Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Audrey N. Schuetz, Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Robin Patel, Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA.

Document Type

Journal Article

Publication Date

9-20-2023

Journal

Antibiotics (Basel, Switzerland)

Volume

12

Issue

9

DOI

10.3390/antibiotics12091465

Keywords

CRE; KPC; Klebsiella; MALDI-ToF mass spectrometry; carbapenem resistance

Abstract

Rapid detection of carbapenemase (KPC) in the species is desirable. The MALDI Biotyper MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical isolates, with whole genome sequences used to assess for and . For the isolates identified as KPC positive by sequencing, the overall sensitivity of the MALDI-ToF MS subtyping module was 239/574 (42%) with 100% specificity. For the isolates harboring , the subtyping module showed a sensitivity of 97% (239/246) and a specificity of 100%. The subtyping module had poor sensitivity for the detection of -positive isolates, albeit exhibiting excellent specificity. The poor sensitivity was a result of being present in only 43% of the -positive isolates.

APA Citation

Cuello, Luz; Alvarez Otero, Judith; Greenwood-Quaintance, Kerryl E.; Chen, Liang; Hanson, Blake; Reyes, Jinnethe; Komarow, Lauren; Ge, Lizhao; Lancaster, Zane D.; Gordy, Garrett G.; Schuetz, Audrey N.; and Patel, Robin, "Poor Sensitivity of the MALDI Biotyper MBT Subtyping Module for Detection of Carbapenemase (KPC) in Species" (2023). GW Authored Works. Paper 3369.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/3369

Department

Public Health Student Works