Hit-and-Run Epigenetic Editing for Vectors of Snail-Borne Parasitic Diseases
Document Type
Journal Article
Publication Date
1-1-2022
Journal
Frontiers in cell and developmental biology
Volume
10
DOI
10.3389/fcell.2022.794650
Keywords
Biomphalaria; DNA methylation; Schistosoma; methylome editing; vector snail
Abstract
Snail-borne parasitic diseases represent an important challenge to human and animal health. Control strategies that target the intermediate snail host has proved very effective. Epigenetic mechanisms are involved in developmental processes and therefore play a fundamental role in developmental variation. DNA methylation is an important epigenetic information carrier in eukaryotes that plays a major role in the control of chromatin structure. Epigenome editing tools have been instrumental to demonstrate functional importance of this mark for gene expression in vertebrates. In invertebrates, such tools are missing, and the role of DNA methylation remains unknown. Here we demonstrate that methylome engineering can be used to modify the CpG methylation level of a target gene in the freshwater snail intermediate host of the human parasite . We used a dCas9-SunTag-DNMT3A complex and synthetic sgRNA to transfect embryos and observed an increase of CpG methylation at the target site in 50% of the hatching snails.
APA Citation
Luviano, Nelia; Duval, David; Ittiprasert, Wannaporn; Allienne, Jean-Francois; Tavernier, Geneviève; Chaparro, Cristian; Cosseau, Celine; and Grunau, Christoph, "Hit-and-Run Epigenetic Editing for Vectors of Snail-Borne Parasitic Diseases" (2022). GW Authored Works. Paper 288.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/288
Department
Microbiology, Immunology, and Tropical Medicine