PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent

Authors

Preethi B. Balakrishnan, Department of Medicine, The George Washington University, 2300 I Street NW, Washington, DC 20037, USA.
Carissa S. Holmberg, The Institute for Biomedical Sciences, The George Washington University, 2300 I Street NW, Ross Hall, Room 561, Washington, DC 20037, USA.
Debbie K. Ledezma, Department of Medicine, The George Washington University, 2300 I Street NW, Washington, DC 20037, USA.
Alberto Bosque, The Institute for Biomedical Sciences, The George Washington University, 2300 I Street NW, Ross Hall, Room 561, Washington, DC 20037, USA.
Rohan Fernandes, Department of Medicine, The George Washington University, 2300 I Street NW, Washington, DC 20037, USA.

Document Type

Journal Article

Publication Date

2-3-2023

Journal

Nanomedicine (London, England)

DOI

10.2217/nnm-2022-0311

Keywords

HIV • HIV latent reservoirs; Prussian blue nanoparticles; latency reversing agents; polyinosinic-polycytidylic acid

Abstract

To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents. A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyIC-PBNPs were stable and monodisperse, maintained the native absorbance properties of both polyIC and PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies. PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free polyIC in CD4 and CD8 T cells. PBNPs function as efficient carriers of nucleic acids to directly reverse HIV latency and enhance immune activation.

APA Citation

Balakrishnan, Preethi B.; Holmberg, Carissa S.; Ledezma, Debbie K.; Bosque, Alberto; and Fernandes, Rohan, "PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent" (2023). GW Authored Works. Paper 2444.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/2444

Department

Medicine