Crosstalk between CD4 T Cells and Airway Smooth Muscle in Pediatric Obesity-related Asthma

Authors

Changsuek Yon, Children's National Hospital, George Washington University School of Medicine and Health Sciences, Washington, DC; and.
David A. Thompson, Children's National Hospital, George Washington University School of Medicine and Health Sciences, Washington, DC; and.
Joseph A. Jude, Rutgers Institute for Translational Medicine and Science, Child Health Institute of New Jersey, Rutgers, The State University of New Jersey, New Brunswick, New Jersey.
Reynold A. Panettieri, Rutgers Institute for Translational Medicine and Science, Child Health Institute of New Jersey, Rutgers, The State University of New Jersey, New Brunswick, New Jersey.
Deepa Rastogi, Children's National Hospital, George Washington University School of Medicine and Health Sciences, Washington, DC; and.

Document Type

Journal Article

Publication Date

2-15-2023

Journal

American journal of respiratory and critical care medicine

Volume

207

Issue

4

DOI

10.1164/rccm.202205-0985OC

Keywords

Th cells; airway smooth muscle; asthma; children; obesity

Abstract

Pediatric obesity-related asthma is a nonatopic asthma phenotype with high disease burden and few effective therapies. RhoGTPase upregulation in peripheral blood T helper (Th) cells is associated with the phenotype, but the mechanisms that underlie this association are not known. To investigate the mechanisms by which upregulation of CDC42 (Cell Division Cycle 42), a RhoGTPase, in Th cells is associated with airway smooth muscle (ASM) biology. Chemotaxis of obese asthma and healthy-weight asthma Th cells, and their adhesion to obese and healthy-weight nonasthmatic ASM, was investigated. Transcriptomics and proteomics were used to determine the differential effect of obese and healthy-weight asthma Th cell adhesion to obese or healthy-weight ASM biology. Chemotaxis of obese asthma Th cells with CDC42 upregulation was resistant to CDC42 inhibition. Obese asthma Th cells were more adherent to obese ASM compared with healthy-weight asthma Th cells to healthy-weight ASM. Compared with coculture with healthy-weight ASM, obese asthma Th cell coculture with obese ASM was positively enriched for genes and proteins involved in actin cytoskeleton organization, transmembrane receptor protein kinase signaling, and cell mitosis, and negatively enriched for extracellular matrix organization. Targeted gene evaluation revealed upregulation of , (tumor necrosis factor), and Cluster of Differentiation 247 () among Th cell genes, and of Ak strain transforming (), Ras homolog family member A (), and , with downregulation of (Protein kinase C-alpha), among smooth muscle genes. Obese asthma Th cells have uninhibited chemotaxis and are more adherent to obese ASM, which is associated with upregulation of genes and proteins associated with smooth muscle proliferation and reciprocal nonatopic Th cell activation.

APA Citation

Yon, Changsuek; Thompson, David A.; Jude, Joseph A.; Panettieri, Reynold A.; and Rastogi, Deepa, "Crosstalk between CD4 T Cells and Airway Smooth Muscle in Pediatric Obesity-related Asthma" (2023). GW Authored Works. Paper 2398.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/2398

Department

Pediatrics