Monitoring Plasma Membrane Injury-Triggered Endocytosis at Single-Cell and Single-Vesicle Resolution
Document Type
Journal Article
Publication Date
1-1-2023
Journal
Methods in molecular biology (Clifton, N.J.)
Volume
2587
DOI
10.1007/978-1-0716-2772-3_27
Keywords
Caveolae; Confocal; Live cell imaging; Membrane repair; Muscle; Muscular dystrophy
Abstract
Plasma membrane injury activates membrane trafficking and remodeling events that are required for the injured membrane to repair. With the rapidity of the membrane repair process, the repair response needs to be monitored at high temporal and spatial resolution. In this chapter, we describe the use of live cell optical imaging approaches to monitor injury-triggered bulk and individual vesicle endocytosis. Use of these approaches allows quantitatively assessment of the rate of retrieval of the injured plasma membrane by bulk endocytosis as well as by endocytosis of individual caveolae following plasma membrane injury.
APA Citation
Bittel, Daniel C. and Jaiswal, Jyoti K., "Monitoring Plasma Membrane Injury-Triggered Endocytosis at Single-Cell and Single-Vesicle Resolution" (2023). GW Authored Works. Paper 2335.
https://hsrc.himmelfarb.gwu.edu/gwhpubs/2335
Department
Genomics and Precision Medicine