An Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy
Frontiers in bioengineering and biotechnology
FRET technique; apoptosis; cancer therapy; drug discovery; xenograft model
In the present study, an efficient drug screening platform is established based on FRET technique. We transfected cancer cells with FRET-based caspase-3 (C3) sensor and validated the cell lines by detecting the change in FRET signal caused by the drug-induced cell apoptosis. Furthermore, the C3 expressing cancer cells were then injected into zebrafish embryos and nude mice to establish the corresponding xenograft models. We found that cancer cell lines expressing C3 were effective in detecting cell death following drug treatment, including the detection of the tipping point of apoptosis. The drug-induced cell apoptosis was also observed in both zebrafish embryos and nude mice xenograft models. Overall, the FRET-based platform, through imaging, is potentially useful to improve drug screening efficiency.
Huang, Shigao; Jiang, Cheng; Mughal, Muhammad Jameel; Wang, Guanyu; and Xing, Fuqiang, "An Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy" (2022). GW Authored Works. Paper 189.
Biochemistry and Molecular Medicine